FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477984
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5620.11757715739438977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG306.3085835E-445.9987454
TATACCG807.081144E-834.4990585
CTAGCCT1650.029.2719294
AGCCTTA756.157239E-527.5992496
GTTAGTC658.651042E-426.5377379
GTTCTAC1101.148599E-625.0954741
GTATAGG1401.38097676E-824.6473431
GCTACTA851.4408218E-424.35482444
GGTACTA851.4418454E-424.35227660
TAGACCC851.4418454E-424.3522764
CCTACGG1001.5615104E-524.1493437
TACACCG1001.5615104E-524.149345
GTGTAAT1301.839453E-723.8889641
GGTCTAG1602.1900632E-923.7230661
GTACAAG902.1212125E-423.0041851
CGAGTCA750.00199043323.0017850
ACTAGGG750.00199043323.0017853
CTACGGG1052.2776527E-522.99937238
GACTCAT1052.2776527E-522.9993727
TAGTACC1352.6532143E-722.999374
Produced by FastQC (version 0.11.5)