FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297626
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5630.1891635811387446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCG200.00734690251.76210858
ACGTTGG200.00735178851.75340349
ATCTACG200.007356676751.74470537
TAGCGGC200.007356676751.74470523
ATTACGA200.007361567651.736018
TACGCGA200.007361567651.736015
TCCGGCA404.99111E-543.1278429
TCGGTGG1350.038.33585451
TCTCGGT1300.037.15629249
CGCCGTA601.3370693E-534.5080759
GTACGCG400.002596696634.4906734
TAGTGTA1500.032.19670540
GCCGTAT652.3120798E-531.85360360
CGATCTA1207.6397555E-1131.62176535
CGTATCA553.2468143E-431.37097262
GGTCGCC1101.0495569E-931.37097256
GACCCGC553.2564328E-431.3551566
CTCCTCG450.00462335530.6583779
CTCGGTG1700.030.4431850
CCGTATC703.835405E-529.57834661
Produced by FastQC (version 0.11.5)