FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13179210
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG734340.5571957651482904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC141550.027.08059969
GCGATCT86250.025.16202769
CGATCTA35850.024.25369
GAGCGAT208000.021.39835769
CGAGCGA339950.018.72558469
ACGAGCG451200.017.58785269
TATACTG24000.017.2494935
TATACCG6050.016.5367035
TAGACTG36700.015.9804555
GTCCTAG41800.015.599711
TAGACCG10750.015.4041985
CTATACT19850.015.2942014
CCTATAC19750.015.1969633
GACGAGC583850.015.14612169
TAGACAG43750.014.9824165
CTAGACT30350.014.8907744
GTATTAG21150.014.8443781
GTATAGG19800.014.8110121
GTATAGA21800.014.7182941
GTCCTAA27350.014.6329431
Produced by FastQC (version 0.11.5)