FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9769875
Sequences flagged as poor quality0
Sequence length75
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG579830.5934876341816042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC73300.025.04189569
CGATCTA17050.022.46250269
GCGATCT49500.021.88688769
CTATACT7750.019.1413654
GAGCGAT102950.019.10328368
GTACTAG7050.017.1280271
CTACACT22900.016.8728624
GTATAAG6450.016.0468561
CTAGACT16750.015.8592294
TAGACAG19900.015.7760745
TACACTG22000.015.3678825
TAGACTG17750.015.35462955
TCTATAC7200.015.3328783
GTGTAGG29500.015.3206661
TAGTACT7250.015.2271344
CCTATAC11450.015.0650553
GTCCTAA17450.014.8283981
TAGACCG12850.014.766255
TAGGACC29000.014.7512864
ACGAGCG213000.014.740808569
Produced by FastQC (version 0.11.5)