FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123396
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15530.13824154616893777No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG450.004619860630.67044869
CCTATAC4000.030.1858443
ATACCGT2500.022.0797736
CTATACT5400.021.0821764
TCCGATG850.00412037220.29390739
GCTCTAG2051.7407729E-920.1940161
TAGTCCG1409.343899E-619.7140835
CCCTATA4200.019.7132052
CTAGAGT2505.0931703E-1119.3189414
GTACACG1258.7444765E-519.3189411
CTAGACT2354.6020432E-1019.084064
CCGTGCG2002.8807335E-818.9748049
TATACCG2406.0936145E-1018.6873075
ACTGATC3700.018.6476278
CCTAGTC2053.824789E-818.511182
GTAGATC3550.018.4654244
CGTACAC1501.694661E-518.3989933
TAATACG2255.5370037E-918.398994
TAAGACT2651.1641532E-1018.2254164
ATAATAC4600.017.9990143
Produced by FastQC (version 0.11.5)