FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210422
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4620.21955879138112938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGT200.00735102551.7494664
CGCCGTA1050.049.28520659
TCTCGGT1550.044.51566749
ACAGCGT404.9883463E-543.1245578
GTGGTCG1600.043.12455754
GTATCAT1150.041.9995663
GTAGATC1400.041.89242644
TGGTCGC1600.040.96832755
CGTATCA1100.040.77230562
GCCGTAT1100.040.77230560
GGTCGCC1700.040.58781456
CTAGTGT1750.039.42816539
CGATCTA1850.039.1617635
TGTAGAT1500.039.09959843
TAGACCA557.366194E-637.6359754
GATCTCG1750.037.45675747
CCGTATC1200.037.37461561
ATCTCGG1950.037.1534648
GTAGGCT655.4234806E-737.153464
CGGTGGT1950.037.1534652
Produced by FastQC (version 0.11.5)