FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13163875
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG528020.40111289418959084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC140950.026.46208269
GCGATCT90850.024.57212469
GAGCGAT215000.022.46740769
CGATCTA37150.021.54728169
CGAGCGA344900.018.2571569
CGATCGA11800.018.12896769
ACGAGCG456050.018.03670369
CTATACT21800.016.9327034
GTCTAAG23550.016.7013241
GTATAGG18900.016.24671
TAGACAG40350.015.8171725
GTACTAG14700.015.4904331
GTCCTAG41950.015.2151671
TACACTG46400.015.16745955
TAGACCG10250.015.1456995
GACGAGC592900.015.09565369
TCTATAC20750.014.9631633
TATACTG26750.014.9601145
CTACACT41000.014.7249294
CTAGACT28950.014.6573524
Produced by FastQC (version 0.11.5)