FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725548
Sequences flagged as poor quality0
Sequence length75
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23520.32416876622911234No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATG200.007358090551.749745
TACACTC1503.45608E-1127.5998635
ATAACGT500.00773325827.5998613
GCTTAAT700.001332875824.6444321
TACCCTA1151.6962822E-623.999885
GCGATCT2800.023.41382669
GTATAAG902.1243827E-423.0014721
ACGTTAA750.001992375422.99988649
CTAATGC1854.820322E-1022.3782673
GGTGTAC953.0627885E-421.7908651
CTCTATA953.0627885E-421.7908652
ATAGACC800.002898496621.5623913
CTAGTAC800.002898496621.5623913
TACACCG1304.917898E-621.2306615
GTCATAA1154.5973622E-521.0013431
CTACACT1507.324834E-720.6998984
ATAAGGG1356.8179215E-620.4443443
GTACCGG1056.0202536E-419.7141886
GTGTAGG2505.0931703E-1119.3212341
CTATACT1258.735692E-519.3199024
Produced by FastQC (version 0.11.5)