FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989053
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15180.15348014717108183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG3400.029.4276981
CGATTAG700.001333390724.64388552
TATAGGG3450.024.0010032
TACTAGG1756.195478E-921.6866192
TAAGACT3500.021.6855244
TTAGACT2401.8189894E-1221.5623114
CTGGACT3350.020.5968344
GTAGACG850.004119992720.29393811
GAGTACG1206.383875E-520.1258411
TAGACTT1206.386358E-520.1248255
TACACGG1056.0225336E-419.7141115
GTCTAAG900.005732479519.1674691
TAAGTAG3250.019.10752533
GCTCTAG1301.1804364E-418.57771
TTAGAGT2053.821333E-818.5120324
CTAAGGT1501.6936558E-518.3998373
GTGCTAC1702.4306064E-618.2654691
CTAGACT1702.4317815E-618.2645454
CTACACT3600.018.2081744
ACTAGGG1150.0011101417.9998423
Produced by FastQC (version 0.11.5)