FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177865
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2130.11975374581845782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG200.00734886551.754
CTAGTAC200.00734886551.753
AGTCTAC200.00734886551.7532
CTTACGG200.00734886551.7530
GAACGGA352.2650698E-549.2857131
ATCCTCG306.297004E-446.040
GCGAATG306.297004E-446.013
CCTTTAG306.297004E-446.026
GATACTA350.001344751139.428575
GTAGCAC350.001344751139.428573
CTCGAAG350.001344751139.4285713
CGGAATG400.00259046334.54
CGGCATC400.00259046334.544
CGTTAAC400.00259046334.545
TGTCGAG400.00259046334.519
AGTGTTA400.00259046334.510
TAGCACT501.8551163E-434.4999964
TAAGCCC501.8551163E-434.4999964
CCAGTAC652.3105818E-531.8461533
TGCCAAC450.004612330.66666612
Produced by FastQC (version 0.11.5)