FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10566427
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259850.2459204043145332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG38750.040.68691312
TGTATAA43850.035.79761511
TATAAGA45000.035.1892813
GTGTATA46950.033.65442310
AGCGATC111200.031.18409569
ATAAGAG51350.031.03928814
ATGTGTA51400.030.8742738
TAAGAGA54750.029.17557315
GATGTGT59750.026.7905797
GAGCGAT174650.024.69524469
TCAGATG66300.024.3522134
TGTGTAT65850.023.9950669
AAGAGAC68400.023.70599416
GCGATCT66250.023.28056569
CTCAGAT72150.023.0949423
AGATGTG70300.022.9665956
CGATCTA27800.021.96849869
CAGATGT74000.021.5385465
CGAGCGA273200.019.07076669
CTACACT29550.017.9791584
Produced by FastQC (version 0.11.5)