FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGGAGAGCTTTCATAGCTAGCTATGTAAAGCTTGTTGAAAGGCACAGCAGAGAGCTTCTCCTGGCATCCCAGC11.4084507042253522No Hit
ATATTGAGTATGTGGTGTGACTGTCACACATGGTGGGACTCCCCACCACCCATGCCCCTGTCTCTTATACACATC11.4084507042253522No Hit
CATGAAAACCACTGCATAAAGCGGGCAGGCGTGGCGGGGATACGAGCGCGCGCCATGTGGTATGGCTGTCTCTTA11.4084507042253522No Hit
AGAGGAAAGACTGGCTGAGAAGCCGGAAGCGGAGGGCCAGGGACCTGGTGGGGGTGCGGGGGCTGTCTCTTATAC11.4084507042253522No Hit
GAGAAATACACACTTTAGGACGTGCAATATGGCGAGAAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTG11.4084507042253522No Hit
GATTAAAGGCTTTTGATTCTTGGGTCGGGGCCTAGACCACTGTTGGGGGGGATTGGGAGGCGCAGAGGCATGGAG11.4084507042253522No Hit
AGGAGGAGGAGGGGGAGGGGGAGGGGGAGGGGGATGTGGAGGGGGCTTTATCTTAAACACATCAGACGCTGCCGA11.4084507042253522No Hit
AGTACACCCTCCTCCTCGCATTTGGCACGACAACGAGACGCCCGCACCATCAGTCTGTCTCTTATACACATCTGA11.4084507042253522No Hit
GAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGAT11.4084507042253522No Hit
GCCGAAAGTGGCGCTGAAGGATTACGGGTACTGACTCGCGAAAAAGTAGATTTAGTTATAAGCGACCTGCGGATG11.4084507042253522No Hit
ATTAATCCCTGCTTCGGTCAGAATTTCGACTTTTTTCGGGTTATTGGTTAACAAGCGGACTTCATTGACGCCAAG11.4084507042253522No Hit
GGTTTTGACGCTGATTCGATATTGTTCCGCTTTTCCATTTAGCACCAGTCGCACGGTCGTAACCCTGTCTCTTAT11.4084507042253522No Hit
GTGATAAATCACCACCAGACAAATACATAACCCTTTGATCTGGTTAATCCATAGCTCTTTTTGTTTCATCAGGAC11.4084507042253522No Hit
GTGCATCCTGTGCCCGCAGGAAGCGGGGGCGGGACGCCGGGAAAACTCGACCAATAGGATCATGGAACGGAATCC11.4084507042253522No Hit
CACCCGAACAGGAGTTTTAGTTAAGCAGGCAGGGCTTACCTACCATGTCTTCTGCCATCTCAATAGCTGTCTCTT11.4084507042253522No Hit
GTAGAATACGCAGCCGGCCCATTCGCGTTATTCTTTATAGCAGAGTACACGAACATTATTCTAATAAACGCCCTA11.4084507042253522No Hit
ACACATAGGTCTGTCCTCCCCCTTGTTTGTTTGATTGCTTGTTAGTTTGAGACAAGGATTAACACCTGTCTCTTA11.4084507042253522No Hit
CCACTGTAGATAGTGGCGGCTTACTGGAGGTTATGGATGGCGGGACTGTAACTGGCGTTGATAAAAAAGCAGGCG11.4084507042253522No Hit
ATACATAACTGCTTTTTATTTTGAAGAAAGTGCTACTGGTTTCTGGGATCCTCAAGTCTTGCCTCTTTGTATCTC11.4084507042253522No Hit
CACATGCAAAGTGGATGCCACACACCTCATTTGTATGACATCCATCATCTGTTCCATGTCATGTTGTAAATTATC11.4084507042253522No Hit
TGTAGCGCCTATCCGCAATCCAGCGCCGTGTTTCAACCGCAGGACGATCAGGATGCCTGCTATGACAACTCCATT11.4084507042253522No Hit
TGATAAACCAGATCAATTAAGCAACACCCCATACAGAAACATTATAAAGCGATCAGCATCACAAAAGACGCCAAA11.4084507042253522No Hit
ATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAA11.4084507042253522No Hit
GGTCAGAAACATTGAGAAGCGAACGGAGAACCAGCTGCTGGTTCAAAGCCTGGTGGAAGCCTGCTCCGGGACCAG11.4084507042253522No Hit
ATTTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCT11.4084507042253522No Hit
TTAAGACGTTCAAAGGAAAGGAGATTTCACTCAGAGGCTCTTTCCGTTTTACCAGCGAATTTAATACCGCATTGT11.4084507042253522No Hit
GTGTCATCCATGCTAACCATAACAGCGGCAGAAGCCAGACGAGCCATCAAGCCGGATACCAGAGACACGGTGAGT11.4084507042253522No Hit
ATCCCAACACAGAGCGCAAAAAGGATAAGGGTTATTGCGGGTAATTGAACATTTAACGGCTTAACCGAAGATGCA11.4084507042253522No Hit
TATTAAAATTCATCGAATGGCATCCTTGCTAACCAACAATGCAAAATCTGTCTCTTATACACATCTGACGCTGCC11.4084507042253522No Hit
GGTTCATTCCTAGCGTCACTATTATCAGGCCTAGTTGGCTTGATGTAGAGCTGTCTCTTATACACATCTGAGGCC11.4084507042253522No Hit
GGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAA11.4084507042253522No Hit
GAGTGAGTGGATGGGTGGCAGAACATAGTCATAGAAGCAGGGGAAGTGAGGTGGGATAGGGGGTTTCCAGAGAGG11.4084507042253522No Hit
GAGCAGGGGCCCGGGCCGAGGGGGCGGAGTTTCCGGGGCGGTCACCTCGCCGCGGGACCCCGCAGGGGACGTCCG11.4084507042253522No Hit
GTATGCCACCAGGTCTTCAATAGTCAATAGCGCCATATTGTGATTATTGGCAAACTCAATAAACTCTGGTGCACG11.4084507042253522No Hit
GTTTTAATGTCATTAGCGGATTTTTACTGGTTGCTAAACCTTCAATGAGTACGCCAACGCCTTTGGCGGCAGTGC11.4084507042253522No Hit
GCCCAGAAGACAGCCCTCCCCAAAGATTAGCCTTACCTTTAGTGACTCTTGCTACTTCTAACCTGGCTCTTATAC11.4084507042253522No Hit
CCTCAGATGTAATGGAGACTCCAGCATGCTTGAGATACCCAGATTGTGGCATGACCACTAAAGACCTGTCTCTTA11.4084507042253522No Hit
GCTCAGGGGCCCCGGGCTCCTGCCTCAACAAGAACACCGAGGGGAAAACCCACCCAGATGACGACGCCCCTATCC11.4084507042253522No Hit
GTTCTAGAGTTCCAAAAACCATTCTCCTGGATTAGGAAAACATTTCAGGAGGATGTGATGGACTGGCAACCCTTG11.4084507042253522No Hit
ATTTACCAGTGGTCGGTTATTAACCCAAATGTTCGTTGCCGGGATGGGGACCAGTGTTATTGCCGGAAATTTTAT11.4084507042253522No Hit
AGTGTGCCTATATGCATTGAGAAGATTAGGAGAGAAGTGAACTTTACATGATTTTCAGTGGACACATTGAAGCTC11.4084507042253522No Hit
GTTAAGAAGGGCTGGCCAATTGGCTGGCCCTTTTTTATCTGTTTGCTGGTGTTTTAAGGGGTAGTAACTGTCTCT11.4084507042253522No Hit
TTCCTGCCCTTTGCGCCAGAGGCTGATTTCTTGGTGGGTAAACACGGTGCGCCGAAAATTGAAATCCAGATGCTG11.4084507042253522No Hit
GTCCAGAGCCAGTCAGTCGGCCAGTTAAATAAGCATTTGAGTTAGCTGCAACAGGTTTAGGAGGAGTGTTCAGGG11.4084507042253522No Hit
GTGCAGGGCCGATTCGCCGCTTCTTTAAGATTGCGCTGCCGCTTATCGCCCCGGTGAGTTTCTTCCTGCTGGTAG11.4084507042253522No Hit
ATCATAATCTAAAAAGAACAGGTGGGAAAGAGCTAGTGACTGGTGAAGATGCTTGAACTAATGAGGCCCTCTCTC11.4084507042253522No Hit
CACTAATATCTCTAACCAAGGTGTTAGAGCTAAAACAAAACAAGAAACACAGATGGCTAATAAATGTCTTCAATA11.4084507042253522No Hit
AACCGACCAGAGTGCAATGCCATATAAATAAGCGTCACAAATAGACACATTCAATAACGAAACTAATACACGCCA11.4084507042253522No Hit
CTTCATAGCGGTATTTCAGGTTGAAACCGCCCATTTTGTTCATTTGGCCCTGAGCGTCGCTCTGTGCGTAACCGC11.4084507042253522No Hit
CAATTGGATGGGTCACACGGCCCCTAGTGGAGGAGCTAGAGAAATTACCCAAGGAGCTAAAGCTGTCTCTTATAC11.4084507042253522No Hit
GACGTAAGCAGCGGGAAATCCCCCCCCCGCCCCCCCCCTTACCCCCGGCCGGCCGCCCCCCCCCCCGGCCCCGCC11.4084507042253522No Hit
AGTTAATAGATATGGTTTCCCATCTACAGAGTTATATCTTGGTAGCTTAATGATTAAGGGCGTGGGCTGTCTCTT11.4084507042253522No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT11.4084507042253522No Hit
GGGCTGGCGCGTTCGGTGCAGGACGGGCTGAAAGCGGCTAACGCCCTGTCTCTTATACACATCTGACGCTGCCGA11.4084507042253522No Hit
GAACCACACAGCCGGAACCGCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGT11.4084507042253522No Hit
CCTCACAGCCACTCTGCATCTCTCTGCTGGTAGCAGAGTTAGTTCTGTCTCTTATACACATCTGACGATGCCGAC11.4084507042253522No Hit
GAAGAAGAGAAAGTCGAAGCTATTGCGATGAGCAAACTTCATAATATGGGTTGTCCGCATTATATGGGGCAGAGT11.4084507042253522No Hit
TGCATAGACTGGCCACTGTCTCTTTAGTCTCTGCTTTGACATGAGCTTGTCTGCAGCTTCAGGAGACTCACCATG11.4084507042253522No Hit
CCCCAATGCTAAGCAATGCACTCTGATACCTGTTTCGGTCAGTTGTTCCACTGTTTTCCTGATATCCATTGCATT11.4084507042253522No Hit
GTGGTGAATAGTTGGTTTAATCAGCCAGGCGTTGAGGAATTTGTTCCACGATCAACCCTGTCTCTTATACACATC11.4084507042253522No Hit
ACCTTATAAACAGGGATCCAAAACAGGTGGTCTAAAGTAGGAGGCCTCAAGGATCCGTAAGTCTGTCTCTAATAC11.4084507042253522No Hit
CTCTTCCTCCTCCTCCCCTCATCTTCTTTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATAC11.4084507042253522No Hit
CAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCTTTCCCGCTACAGCTGTCTCTTATACA11.4084507042253522No Hit
CTAGGCACTGGAAGAGCTGAGCCACTGAGGGCAAGGGTAGCAGATCCCAGAGCCAGAGCAGAGGACTCAGCTGCA11.4084507042253522No Hit
GACGGGCGGTGTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTACTACTAAATCCTCCT11.4084507042253522No Hit
CGATATGTTCCTGTAGCGCGCTTTCGCGGGTGTAGTCATCTTCGGCGTTGAAATAGCGTTCATAGGCGACCAGCG11.4084507042253522No Hit
CTCCTCCTCCGACCTTCCGACTCCGCCTCCCTCTGTAGTTCTCCGAAAGCCCTCCTAGGCCCCGCCCCTGTCTCT11.4084507042253522No Hit
AAACTAAAACGCTGTTGATCAAAAACCATCCCTTTCAACTCATGGAGATAATCGCTCATCTTGGCCAGACCGATC11.4084507042253522No Hit
ACCTAACTCGCCGGCAGGGCACGCTATCACGTCGACCACGTGCATAATCCTGAAGACGTCCAGCTCAGTAAAAGG11.4084507042253522No Hit
AAGTGAAACACGTGAGGGCACAACCGGGCGCCTCACACATCCACAAGGACCACGCGAACCACTGAGAAAAGTGCG11.4084507042253522No Hit
GTCGTAACAAGGTAAGCATACTGGAAAGTGTGCTTGGAATAATCATAGTGTAGCTTAATATTAAAGCATCTGGCC11.4084507042253522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCCG50.069.08
CCGGAAC50.069.012
CAGCATG50.069.034
CCGTGCC50.069.021
AACCGCC50.069.016
GCCTTCG50.069.025
CGATAAA50.069.047
CACACAG50.069.05
AATTTTT50.069.059
TTCGCGC50.069.028
GCGATAA50.069.046
GGCAATT50.069.056
TTTCCAC50.069.064
ACCACAC50.069.03
TAAAAGC50.069.041
TAAAACG50.069.050
CCGCCGT50.069.018
TTTTTTC50.069.061
TCGCGCA50.069.029
CAATTTT50.069.058