FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8161662
Sequences flagged as poor quality0
Sequence length75
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201020.2462978741339693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC79250.029.08239769
CGATCTA18400.025.1269669
GCGATCT49100.024.52431969
TGTATAA10900.024.37137211
GTATAAG11150.023.51551212
GAGCGAT119200.022.34570569
GTGTATA11700.022.11521310
TATAAGA12800.020.48421713
TAGACTG11950.020.2088015
ATGTGTA13650.018.9556668
CTAGACT14300.018.8174614
CCTATAC9650.017.8749623
TACACCG13550.017.8225235
TAGACCG10950.017.6434825
TACACTG16900.017.5558645
GTCCTAG25950.017.5498581
CTACACT20950.017.2905064
GTGTAGG24200.016.9655761
TAGACAG14750.016.8403425
TATACCG3950.016.5946065
Produced by FastQC (version 0.11.5)