FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2004736
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAA9650.030.38705311
ATGTGTA10000.028.9785218
GTATAAG10300.027.4646212
GTGTATA10350.027.33194210
TATAAGA10900.026.26930213
AAGAGAC11250.026.06598716
ATAAGAG11250.025.75868614
CTCAGAT12700.025.534773
TCAGATG12000.025.2993414
CAGATGT12650.023.4539365
TGTGTAT12250.023.0927019
TAAGAGA12400.022.81392315
GAGACAG13650.022.4939218
AGAGACA13650.021.98843817
CCTCAGA15300.021.6486572
AGATGTG13650.021.4829586
GTCTAGA2451.8189894E-1221.1240061
GATGTGT13800.020.9989287
TCTATAC4950.020.2115963
GTACTAG1056.0221425E-419.715741
Produced by FastQC (version 0.11.5)