FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114677
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1690.1473704404544939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGG150.002350128769.019
ATGCGAC150.002350128769.022
CCGGTAT150.002350128769.03
CGGTCAT150.002350128769.011
GTCTTCG150.002350128769.041
CGGTATA150.002350128769.02
TATACGC150.002350128769.05
TCCGATT200.00734223951.7519
TCTTAGT200.00734223951.759
GTAGGTA200.00734223951.7544
GTAATAG200.00734223951.751
TCGGACT200.00734223951.753
CTAGTCC200.00734223951.753
CTGCATA200.00734223951.7558
TTGGTAC200.00734223951.7524
CATGTAT200.00734223951.753
CTTTCGC200.00734223951.7549
TGTCGTG200.00734223951.7540
AGGTCTT200.00734223951.7539
TTGGCTT306.287592E-446.05
Produced by FastQC (version 0.11.5)