FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8935561
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150810.16877507746855513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG25400.029.47309912
AGCGATC56750.027.54260669
TGTATAA29250.025.94756511
TATAAGA30850.024.48999613
GAGCGAT84000.024.44051769
GTGTATA32100.023.42886410
CGAGCGA128700.020.83124269
ATGTGTA39500.019.3887968
ATAAGAG40500.019.16581314
ACGAGCG171650.018.61400869
GATGTGT43450.017.8643687
TAAGAGA45100.017.36445815
CTATACT17150.017.299624
TATACTG17000.017.2494265
GTGTAGG31100.016.5291291
TAGACAG33250.016.2896845
GACGAGC230650.016.27601869
GCGATCT40100.016.26260669
CTAGACT23650.016.1917614
GTCTTAC17950.015.5684251
Produced by FastQC (version 0.11.5)