FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5013643
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75330.15025002777421528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG18900.039.06309512
TATAAGA21700.033.86370513
TGTATAA21950.033.6351911
GTGTATA23200.031.67424410
ATGTGTA25200.029.570838
ATAAGAG25650.028.7833314
GATGTGT27850.027.3764677
TAAGAGA30250.024.86280315
AGCGATC22600.023.51095469
TCAGATG32650.022.8236564
TGTGTAT34400.021.461999
GAGCGAT32700.021.41934869
AAGAGAC36350.021.25975416
CTCAGAT35850.020.9788653
AGATGTG38050.020.2192386
CAGATGT37100.020.1790375
CTATACG1801.984863E-719.166474
ACGAGCG65600.018.82942869
CGATCTA5150.018.7589669
CGAGCGA50150.017.88794169
Produced by FastQC (version 0.11.5)