FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468214
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGTA7150.028.9515788
GTGTATA7500.027.60050610
TGTATAA7750.026.71016511
TCAGATG7750.026.7083454
AAGAGAC8350.025.61723516
GTATAAG8400.024.64330712
TATAAGA8600.024.47137813
CTAGCCT4250.023.5400034
TAAGAGA8850.023.39025715
TAGACCG902.126481E-423.0004225
CAGATGT9450.022.6353345
GATGTGT9500.022.5162037
CTCAGAT9650.022.16473
AGATGTG10050.021.627266
ATAAGAG9950.021.1511414
GAGACAG10650.021.05672318
TGTGTAT10050.020.5973919
TTAGAGT2102.3519533E-919.7133054
AGAGACA11000.019.4458117
GTCTAGG4350.019.0335331
Produced by FastQC (version 0.11.5)