FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149838
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1750.11679280289379197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC453.0047886E-853.66666855
ATGTGTA653.6379788E-1253.0769238
ATAATCG200.00734661451.7534
CCTATAG200.00734661451.752
TAGTCTT200.00734661451.755
GTATAAG707.2759576E-1249.28571312
CTAGTGT451.850507E-646.039
TAGTGTA451.850507E-646.040
ATAAGAG856.548362E-1140.58823414
TATAAGA856.548362E-1140.58823413
ACTACAC350.001344072539.428573
GGACACA350.001344072539.428576
CCACGAC350.001344072539.428573
CGTATCA459.974031E-538.33333262
TGTATAA901.200533E-1038.33333211
TCAGATG901.200533E-1038.3333324
GTAGATC557.3514802E-637.6363644
CTACCCT557.3514802E-637.636364
TAGATCT557.3514802E-637.6363645
GTGTATA952.1645974E-1036.31579210
Produced by FastQC (version 0.11.5)