FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15047646
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267520.17778196004876776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG71550.045.66129312
TGTATAA80750.040.63004311
GTGTATA80700.040.35596510
TATAAGA81600.040.2489613
ATAAGAG89600.036.5397914
ATGTGTA92500.035.4312828
TAAGAGA99950.033.10240615
GATGTGT100450.032.833077
AGCGATC143600.030.44251669
TCAGATG110100.030.1433354
CTCAGAT113500.029.6962913
AAGAGAC115650.029.11526116
AGATGTG114150.029.0439286
TGTGTAT112800.028.9634449
CAGATGT118750.027.8317015
GAGCGAT220350.024.38001369
GCGATCT86500.024.29179469
CGATCTA35600.022.58209869
CCTCAGA176300.020.8021892
AGAGACA165550.020.38103117
Produced by FastQC (version 0.11.5)