FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9588470
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335120.34950310112040817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG20550.030.5539112
AGCGATC91150.029.71461169
TGTATAA22200.028.4385611
GCGATCT52350.027.08832669
TATAAGA23650.026.54895613
GTGTATA26700.023.64554210
GAGCGAT138050.023.44355669
ATGTGTA27150.023.2532658
ATAAGAG29400.021.70859114
CGATCTA17950.021.1439169
CTAGTAC8850.019.490793
TAGACCG11200.019.097695
TAAGAGA33800.018.9854815
CGAGCGA223950.018.47247969
TCAGATG36450.018.4560934
GATGTGT35850.017.8989057
ACGAGCG289200.017.44239469
CTAGACT19350.016.9373444
GTATTAG11550.016.728211
TAGACAG25600.016.710485
Produced by FastQC (version 0.11.5)