FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790252
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGTA18550.028.0824768
GTGTATA18500.027.78578210
TGTATAA19100.026.91292811
GTATAAG19150.026.66250812
ATAAGAG19900.025.31091314
CTCAGAT22200.024.8639053
TATAAGA20550.024.84608513
AAGAGAC20750.024.77351816
TCAGATG22400.023.4098114
TAAGAGA24350.021.25258615
GATGTGT24400.021.2081977
CAGATGT25100.020.4792885
TGTGTAT25250.020.3578999
AGATGTG26350.019.6387126
AGAGACA26550.019.361617
CCTATAC12100.019.1025683
GAGACAG28150.018.99646618
GTCTTAA7950.018.6608871
CCTCAGA30250.018.590592
CCCTAGT6450.018.1865441
Produced by FastQC (version 0.11.5)