FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32121
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440.1369820366738271No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAA150.00233573868.9922131
GAGCGAT308.849989E-657.58320269
ACTCCAA252.5310734E-455.1937748
GTTGAGG200.00729764551.7441621
TGGGTCT200.00729764551.7441660
GGACTCT200.00729764551.744164
TGTTCCA200.00729764551.744165
AGCAGTA200.00729764551.7441635
GACTCTA200.00729764551.744165
GTAAAAC200.00729764551.744163
GAAGGGT200.00729764551.744164
TACCGCA200.00729764551.744167
TCTGCTG200.00729764551.7441641
CTCCAAG400.002560490534.49610549
CGAGCGA553.160545E-431.4090268
CAGCTGT450.00455927130.66320661
CTGGGGC450.00455927130.6632064
CTGCTGC500.007629467627.5968861
GGCTGGG500.007629467627.59688655
ACGAGCG800.002815325521.593767
Produced by FastQC (version 0.11.5)