FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060429
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAGA3750.030.3600410
CTAGGAA1353.0559022E-1028.1111512
CTATACT2500.026.2200344
GTATAGA1850.026.1093731
TAGACGT809.5610565E-525.8750364
CTCAGAT4000.025.87503611
GGGGTCC1251.264325E-724.8400336
CCTAGAC700.001333767524.6428933
GCCTCAG3750.023.9200339
GGACTTA1301.8470746E-723.8846476
AGGGGTC1452.0145308E-823.7931395
TTAGAAC1602.2009772E-923.7187823
ATGTGTA3950.022.7078216
TGTGTAT3350.022.6556817
AGAGACA4450.022.48105625
TCTCCGC4300.022.4651474
AAGAGAC4400.021.95250524
CGCCTCA4450.021.7058498
GGTCCTA1455.296897E-721.4118028
TAAGAGA4200.021.35515823
Produced by FastQC (version 0.11.5)