FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136871
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT5300.031.89523111
CCTCAGA5850.030.66570910
GTCTCCG2200.028.2251513
CGCCTCA5350.027.7268858
TCTCCGC4550.027.294654
TCCGCCT4700.027.1575326
GCCTCAG4850.027.0300859
AAGAGAC5600.026.4890824
CTCCGCC5550.026.1061445
CCGCCTC5750.025.798067
TAAGAGA5050.025.27532623
GAGACAG5350.023.2142326
AGAGACA6650.022.82635525
ATAAGAG6250.020.42246422
CGTGCGA1701.08524546E-720.29348410
TATACTG3200.019.4047895
GACTAGT900.00573541419.16606711
TATAAGA7050.019.0853521
AGATGTG5650.018.92861414
ACGAGCG9450.018.62092269
Produced by FastQC (version 0.11.5)