FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88459
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGA150.002351172768.9790810
ATAGTAT150.002351172768.979088
CGAGCAA309.073163E-657.48256710
CCATGTC252.5580396E-455.183273
TTTAGTA200.007329100751.76357337
GAGAGGT200.007329100751.76357334
CGGCTAG200.007329100751.76357343
ACCGGAG200.00734551851.7343127
GGGCTCA200.00734551851.7343111
GGGTAAC200.00734551851.7343116
TCCTCGG200.00734551851.7343127
CGATTCT200.00734551851.734318
ACCTAAC200.00734551851.7343140
GGCCTAC200.00734551851.734311
CCGAGCA352.2597995E-549.2707759
ACATCCC306.2891433E-445.98605316
GGGATCG306.2891433E-445.98605321
CGCTTCG306.2891433E-445.98605331
CTCCATG404.9734022E-543.1119271
ATATGGA350.001339357639.43891559
Produced by FastQC (version 0.11.5)