FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8047296
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86870.10794930371642848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC75800.025.58181869
GAGCGAT114500.023.83610269
GCGATCT45600.021.7917469
CGAGCGA176850.019.33449769
ACGAGCG229450.018.37584969
TCTAGAC26750.018.1846793
TAGACTG29450.018.1575285
CTACACT28550.017.6423744
CGATCTA24350.017.1455269
GTATAGA14250.016.7062721
TACACCG8550.016.5435245
TAGACAG29950.016.472125
CTAGACT28100.016.4516244
TACACTG34550.016.1762285
TAGGACT28600.016.164014
TAGGCCG19500.015.9227545
GTGTAGG34350.015.8699561
TATACTG15900.015.6223255
GTATTAG11150.015.4717881
GACGAGC300400.015.3681169
Produced by FastQC (version 0.11.5)