FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650616
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC27850.026.14046969
GAGCGAT44950.025.33030169
GCGATCT16750.021.83469869
CTACACT16200.018.5277634
ACGAGCG87600.018.1573769
CGAGCGA68100.018.1381169
CTATACT9000.018.0166534
TAGGCCG13750.017.8145315
TATACGG2156.6047505E-817.651155
TACACTG18850.017.5702785
TCTATAC8350.017.3532813
GTATAGG8250.017.146181
TATACAG13700.016.872255
TAGGACT16900.016.3313474
CTAGGAC23300.016.287543
TAAGCCG4450.016.2808865
CTAGTAC7300.016.0684813
TAGTACT8350.015.7005874
GTCTAGA15400.015.682481
TATAAGA10800.015.65276621
Produced by FastQC (version 0.11.5)