FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351449
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3650.10385575147460942No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCACG200.007355046551.749579
TAGACCG306.3057E-445.99962219
TCTCCGC1700.036.529114
AACCGAT400.002593998634.4997147
CGCCTCA2150.033.69748
CGTCTCC1101.0495569E-931.3723072
GTACGGC450.004618556230.66641457
ATAGGGC703.8437105E-529.5711863
GGATAGG953.185396E-729.0606611
CCGCACA605.4149E-428.74976325
GTATAAG2600.027.86515220
TAACGTG500.00772804227.59977359
CGGACTT500.00772804227.59977318
GTCTCCG1503.45608E-1127.5997713
GCGATCT3000.027.59977169
CGTAACA756.151876E-527.59977116
TTATAGC1501.036824E-925.2997913
TGCCCTA700.001332069624.6426545
TACTGAC700.001332069624.6426547
GTCCTAA851.4378178E-424.3596731
Produced by FastQC (version 0.11.5)