FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136496
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG451.00151694E-438.33364
CCTCAGA5950.036.5296710
CGCCTCA5450.035.4482278
CTCAGAT4800.035.21899811
ATGTGTA4950.031.36385516
ATAAGAG5400.031.30302222
AAGAGAC6150.030.8511624
TAAGAGA5500.030.73387523
AGAGACA6600.029.79304325
GTCTCCG3250.029.7232863
GCCTCAG5950.029.5716389
TGTGTAT4800.029.46895617
GTGTATA5850.027.71814218
CTAAGCT1254.150934E-927.6001954
AGATGTG5900.027.48324414
TATAAGA6950.026.80594321
GATGTGT5800.026.7674315
GAGACAG6200.026.70633926
TCTCCGC6400.025.8751814
TGTATAA7050.025.44698719
Produced by FastQC (version 0.11.5)