FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6558178
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG9950.028.4327221
TTGACGG11200.023.1024331
TAGACTG26750.023.0852035
AGCGATC54250.022.2607169
GAGCGAT75450.022.04238169
ACGGCTA12250.021.40352234
CGGCTAT12350.020.9508735
TCTAGAC29100.020.3909113
CTAGACT28600.020.3855234
CTTGACG13300.020.23286430
GCGATCT33850.019.77487669
TAGACAG23450.018.8309235
TACACTG29550.018.3293325
CTATACT18500.018.0884424
CGAGCGA110000.017.50298969
GACTGTG43500.017.2093727
TAGACGC11050.017.1712364
ACGAGCG139300.017.09104769
CTACACT26100.017.051024
TGACGGC15800.016.8131132
Produced by FastQC (version 0.11.5)