FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819770
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG3000.027.6010071
GTCCTAC20700.025.1675851
TACACCG6700.024.2015155
CCGTGAT12150.023.5670927
TTCCGTG12850.023.088725
GTAGGAC19950.022.8270913
CGTGATT12700.022.00317828
TCCGTGA13700.021.90761826
CTACAGT25100.021.4422514
TAGGACG20700.021.0000214
CGACTTG9750.020.87731464
TTTCCGT13200.020.64701724
TCCTACA25750.020.6337642
CTACACT12050.020.614134
AGCGATC31900.020.55006869
GTCCTAA20350.019.8361051
TACAGTG28400.019.6795965
TGTAGGA24850.019.5761662
CCTACAG25850.019.4855123
GACGACT10950.019.2195462
Produced by FastQC (version 0.11.5)