FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12583056
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129480.10290028113997109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC135350.024.39504669
GAGCGAT193700.021.03624569
CGAGCGA291850.019.78990469
GCGATCT86450.019.67568669
ACGAGCG388550.018.98491969
TATACTG26950.018.8173395
CTATACT28200.017.9832364
CTACACT48200.017.7502424
GACGAGC522600.017.22473769
TACACTG57350.016.6627245
CTAGACT45200.016.5623114
TAGGACA42300.016.3113254
TAGACAG49300.016.3045445
TAGACTG45300.016.0688175
TACACCG15100.015.5357285
GTGTAGG57900.015.3735291
CGATCTA49400.015.2955569
CCTATAC27750.015.1668883
TAAGACA42850.015.0553354
GTCTAGG50300.015.0213361
Produced by FastQC (version 0.11.5)