FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483597
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG1401.3867975E-824.6476251
GTCCTAC16900.024.29761
TAGGACG16950.023.6099574
AGGACGT19050.021.9127715
GTAGGAC15700.021.3146923
TCCTACA20100.020.0859762
CTACAGT21300.019.9219784
TAGGACT6450.019.7901444
GGACGTG18850.019.7660266
TATACCG4550.019.7137345
CTACACT8550.019.3678784
TGTAGGA19050.019.2005652
ACGTGGA11450.018.9820028
CCTATAC6000.018.9744683
TCCGTGA11200.018.78927426
CCTACAG20600.018.4218143
CTAGACG1501.6956163E-518.3994833
GTCCTAG5300.018.2299421
TACACTG14450.018.144825
GACGTGG12250.018.0239857
Produced by FastQC (version 0.11.5)