FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344253
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC14400.023.2409341
GTAGGAC15700.021.0955053
TGTAGGA18600.019.6624322
TCCTACA17750.019.437752
CTACAGT19250.019.355814
TAGGACA9850.018.563424
CGTACAC5500.017.5636063
TAGGACG15400.017.4739954
TACACCG11650.017.4723345
CCTACAG20150.017.463993
TAATACT12350.017.0404574
TACAGTG23150.016.9894265
CTGTAGG20600.016.581061
CTAGACT5850.016.5127934
CGAGCGA32950.016.43999169
CCTACAC11000.016.3090633
TAAGGGT7200.016.291644
CGATCTA8000.015.95765569
GTATAGG6500.015.9240031
ACGAGCG43350.015.75917269
Produced by FastQC (version 0.11.5)