FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184178
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT6500.019.1068884
GTCCTAC7400.018.6508521
TATACTG5750.018.5992165
CAGGACT7800.018.576144
GTAGGAC8000.018.1117363
TAGGGAG4200.018.0706675
TCTATAC5200.017.9127083
CTATACT4700.017.616284
ACTGCGC12000.017.5367628
TAGGACG8350.017.3525624
GAGCGAT20600.016.74878569
TACACTG9450.016.4278815
TTAGGAC7500.016.0993213
TAAGACT5600.016.0171814
ACGAGCG31200.015.92422869
TAGGACA5650.015.8754384
TCTACAC7400.015.8506843
TCCTACA8200.015.5689122
CGAGCGA24150.015.42968769
CCTACAC6100.015.2698493
Produced by FastQC (version 0.11.5)