FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13916182
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC126000.024.56257469
GAGCGAT180150.021.60363469
GCGATCT79250.020.11382569
CGAGCGA267650.019.60712869
ACGAGCG356500.019.35630469
CTATACT31700.017.739374
TATACCG5900.017.5421895
TATACTG29950.017.0482355
CCTATAC33300.016.9906273
TACACTG68350.016.7072125
CTACACT56250.016.620984
GACGAGC479450.016.58025469
GTCCTAC40500.016.5265251
CTAGACT53050.016.192874
GTATAGG29200.015.5964551
TAGACTG57900.015.1345565
TAGGCCG24400.015.1289395
CGATCTA46600.014.95606869
TCTAGAC50600.014.93149953
TAGACAG55450.014.8699985
Produced by FastQC (version 0.11.5)