FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7730570
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC78350.023.11911469
GAGCGAT110300.021.92771569
GCGATCT50650.019.89089469
ACGAGCG214250.019.3085369
TACACCG8300.019.1201525
CTACACT31900.018.3851414
TACACTG39150.017.8885785
CGATCTA29150.017.75430369
GACGAGC290900.017.6011569
CGAGCGA159700.017.434969
CTAGACT27000.017.3773634
TATACTG16150.017.3031065
TAGACTG29600.016.7834935
GTATTAG12550.016.4944861
CCTATAC15650.016.3127123
TCTAGAC27650.016.0954573
CTATACT16200.015.9718424
GTCCTAG31700.015.6723631
GTGTAAG25300.015.4095211
CCTACAC38250.015.3329693