FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47425316
Sequences flagged as poor quality0
Sequence length20
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGGATGGGGTTTGGG29225676.162461837892656No Hit
GTGGGGTTATGTGGTTTGGG27487915.79604150660799No Hit
GTGGGGTTATGGGGTTTGGG25336315.3423597641394736No Hit
GTGTTTGGTTGGGGTTTGGG23574164.970796609979362No Hit
GTGTTTGGATGTGGTTTGGG22488264.74182607449574No Hit
GTGGGGTTTTGGGGTTTGGG20754624.376274477538536No Hit
GTGGGGTTTTGTGGTTTGGG20241994.2681824197017475No Hit
GTGGGGTGATGGGGTTTGGG17587633.708489786341118No Hit
GTGGGGTGTTGGGGTTTGGG17382523.6652407334513066No Hit
GTGGGGTGATGTGGTTTGGG14460013.04900656855929No Hit
GTGTTTGGTTGTGGTTTGGG14032962.9589597252235493No Hit
GTGGGGTGTTGTGGTTTGGG11801322.4884009207234383No Hit
GTGGGGGTATGGGGTTTGGG1386350.29232277545604546No Hit
GTGGGGGTTTGGGGTTTGGG791530.16690031121774707No Hit
GTGGGGGTATGTGGTTTGGG610660.12876245252641016No Hit
GTGTTTGGATGGGGGTTGGG584540.12325484557656928No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTT1050.014.0102181
CCCCGTT1600.014.0102181
CCCCGGT2750.014.0102181
CGCCGTT559.107025E-714.0102181
CTCCGGT3950.014.0102181
CCGCGTT401.7452364E-414.0102181
CCGCGGG1600.014.0102181
CCTATGG300.005911239414.0102171
CTCCTGT14650.014.0102171
CTCCTGG18000.014.0102171
CCTCGTT3550.014.0102171
CCGCTTG1200.014.0102171
CCCATTG300.005911239414.0102171
CCCCGGG4850.014.0102171
GCCCGTT3300.014.0102171
CTCCGTG7050.014.0102171
GTACGGA300.005911239414.0102171
CCGCGGT905.456968E-1214.0102171
GTCCGGT3016300.014.0085911
ACTGACA2750.013.9998463
Produced by FastQC (version 0.11.5)