FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8865510
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT183800.20732027824682392No Hit
GGGGGGGGGGGGGGGGGGGG148320.16730001996501048No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGG17750.014.0075121
GTCCGTG7550.014.0075121
GGCCGTG4150.014.0075121
CCGCTTT350.001014770514.0075121
CCGCTTG704.8748916E-914.0075121
GTCCGGT4150.014.0075121
CCGCTGT1350.014.0075121
CGGCGTG559.124469E-714.0075121
CCCCGTG4150.014.0075121
CTGCGGT2100.014.0075121
GTCCTGG17750.014.0075121
TCGCTTT559.124469E-714.0075121
TCGCTTG350.001014770514.0075121
CGTCGTG559.124469E-714.0075121
TCCCGTT2300.014.0075121
TCCCGTG3950.014.0075121
TCCCGGG3100.014.0075121
GCTCTTG8800.014.0075121
CGTCTGT1550.014.0075121
CGCCGGT704.8748916E-914.0075121
Produced by FastQC (version 0.11.5)