FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515589
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT9980.19356502950993912No Hit
GGGTTTGGGTTTGGGTTTGG9100.17649717119643749No Hit
GGGGGGGGGGGGGGGGGGGG8610.16699347736278314No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGG505.2006562E-614.0127191
TTCCTTG505.2006562E-614.0127191
GTCCTTG1000.014.0127191
GTCCTGG1000.014.0127191
CTGCTGG652.7495844E-814.0127191
TCTCTTG652.7495844E-814.0127191
CCCCTGG505.2006562E-614.0127191
CTTCTTT505.2006562E-614.0127191
CTCCTTT559.0385583E-714.0127181
CTCCTTG453.000915E-514.0127181
CTCCTGT401.737323E-414.0127181
GTCCGTG350.001009652714.0127181
CCTCGTG401.737323E-414.0127181
TTCCTTT300.00589378714.0127181
TTCCTGT852.5465852E-1114.0127181
TTCCTGG350.001009652714.0127181
TGCCTGT453.000915E-514.0127181
GTCCTTT801.4733814E-1014.0127181
GGCCTTG350.001009652714.0127181
GTCCTGT704.809408E-914.0127181
Produced by FastQC (version 0.11.5)