FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677151
Sequences flagged as poor quality0
Sequence length20
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG11030.16288833657485555No Hit
GTGTGTGTGTGTGTGTGTGT9760.14413328784864823No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG704.8457878E-914.0074631
CTCCTGT704.8457878E-914.0074631
CTCCTGG1050.014.0074631
CCTCGGG300.005910990314.0074631
TTCCTTT601.5844853E-714.0074631
TGCCTGG652.76832E-814.0074631
CCTCTTT300.005910990314.0074631
CCTCTGG704.8457878E-914.0074631
GGCCTGG652.76832E-814.0074631
GCTCGTT300.005910990314.0074631
CCCCTTT350.001013096914.0074631
CCCCTGT300.005910990314.0074631
TCTCTGG652.76832E-814.0074631
GCCCGTG300.005910990314.0074631
CTCCGTG300.005910990314.0074631
CGTCTGT300.005910990314.0074631
TCCCTTG300.005910990314.0074631
TCCCTGT601.5844853E-714.0074631
TCCCTGG652.76832E-814.0074631
GCCCTGT601.5844853E-714.0074631
Produced by FastQC (version 0.11.5)