FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3835469
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT74370.19390066768887976No Hit
GGGGGGGGGGGGGGGGGGGG58620.1528365892150347No Hit
GGGTTTGGGTTTGGGTTTGG45900.11967245726663414No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTTG4050.014.0076581
TTCCTGT3750.014.0076581
TTCCTGG3750.014.0076581
CTTCGTG3450.014.0076581
GCTCTGT5150.014.0076581
TCCCTGT3150.014.0076581
GCCCTGT3750.014.0076581
CTCCTTT2550.014.0076571
CGCCTTG758.54925E-1014.0076571
TTCCGTT1150.014.0076571
TGCCGTT401.7472854E-414.0076571
TGCCGTG652.7810529E-814.0076571
CTCCTGG5950.014.0076571
TTCCGGG758.54925E-1014.0076571
GTCCGTT1150.014.0076571
CCTCGTT1200.014.0076571
GGCCGTG1500.014.0076571
CCGCTTT350.001014506314.0076571
CCTCGGT1500.014.0076571
CCTCGGG1150.014.0076571
Produced by FastQC (version 0.11.5)