FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5504724
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT125060.2271866854723325No Hit
GGGTTTGGGTTTGGGTTTGG89780.16309627875984337No Hit
GGGGGGGGGGGGGGGGGGGG82660.15016193364099636No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTG758.54925E-1014.0080541
TGCCGTT704.8712536E-914.0080541
CGCCTGT1400.014.0080541
CGCCTGG1400.014.0080541
TGCCGGT652.7810529E-814.0080541
CCTCGGT1950.014.0080541
TCGCGTG350.001014387814.0080541
CTGCGTT652.7810529E-814.0080541
GTCCTTT5750.014.0080541
CCCCGTT1450.014.0080541
TCTCGGT1400.014.0080541
CTTCGGT2000.014.0080541
CGTCGGT505.240734E-614.0080541
TCCCGTG1950.014.0080541
CCCCTGG5800.014.0080541
TCCCGGT1000.014.0080541
GCCCGTG2800.014.0080541
GCCCGGT1450.014.0080541
GCCCGGG2000.014.0080541
CGTCTTG704.8712536E-914.0080541
Produced by FastQC (version 0.11.5)