FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721235
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT55850.20523769538463235No Hit
GGGGGGGGGGGGGGGGGGGG45530.16731373806378352No Hit
GGGTTTGGGTTTGGGTTTGG32700.1201660275573407No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTT401.747779E-414.0069131
TTCCGTT350.001014780614.0069131
TTCCGTG1050.014.0069131
TTCCGGT1050.014.0069131
TGCCGGT350.001014780614.0069131
GGCCGTT401.747779E-414.0069131
CCGCTTT350.001014780614.0069131
GTCCGGT1400.014.0069131
GGCCGGG1250.014.0069131
TGCCTTG1250.014.0069131
TTCCTGG3200.014.0069131
CTGCGTT350.001014780614.0069131
CCCCGTT704.8730726E-914.0069131
CCTCTGG3500.014.0069131
CCCCGGG350.001014780614.0069131
TCTCGGT1050.014.0069131
TCGCTGT401.747779E-414.0069131
GCGCTTG401.747779E-414.0069131
CCCCTTT1250.014.0069131
TCTCTTT2350.014.0069131
Produced by FastQC (version 0.11.5)