FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9537545
Sequences flagged as poor quality0
Sequence length20
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT186750.19580510498246667No Hit
GGGGGGGGGGGGGGGGGGGG175800.18432416308389632No Hit
GGGTTTGGGTTTGGGTTTGG152350.1597371231275973No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGGG1900.014.0079171
CCTCGTG6350.014.0079171
GTCCTTT8900.014.0079171
CCCCGTG4250.014.0079171
CTCCTTT7400.014.0079161
CGCCTTT905.456968E-1214.0079161
CGCCTTG1350.014.0079161
CGCCTGT1750.014.0079161
GTCCGTT3450.014.0079161
CCTCGTT3500.014.0079161
GGCCGTT2500.014.0079161
CCGCTTT601.5912701E-714.0079161
GTCCGGT5000.014.0079161
CCTCGGT4300.014.0079161
CCGCTGG2900.014.0079161
TTCCTTG7450.014.0079161
GTCCTTG15650.014.0079161
CCCCGTT1850.014.0079161
TCTCGTT2300.014.0079161
TCTCGTG4200.014.0079161
Produced by FastQC (version 0.11.5)