FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016167
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT52150.25865912893128395No Hit
GGGTTTGGGTTTGGGTTTGG50720.2515664625003782No Hit
GGGGGGGGGGGGGGGGGGGG44020.2183350883136169No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGG1950.014.0102771
CCTCTGG2950.014.0102771
CGCCTTG559.0941467E-714.0102761
TTCCGTT350.001012780914.0102761
CTCCTGT2850.014.0102761
CTCCTGG4050.014.0102761
TGCCGGT401.7438672E-414.0102761
GTCCGTG1750.014.0102761
CCTCGTG1300.014.0102761
GGCCGTT505.228183E-614.0102761
GGCCGTG1100.014.0102761
GTCCGGT1050.014.0102761
CCTCGGT652.771776E-814.0102761
CCTCGGG1300.014.0102761
GGCCGGT652.771776E-814.0102761
TTCCTTT1250.014.0102761
TGCCTTT758.5128704E-1014.0102761
TTCCTGT1600.014.0102761
TTCCTGG1700.014.0102761
TGCCTGT1300.014.0102761
Produced by FastQC (version 0.11.5)