FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878816
Sequences flagged as poor quality0
Sequence length20
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT35520.1890552347861632No Hit
GGGTTTGGGTTTGGGTTTGG28940.1540331783421048No Hit
GGGGGGGGGGGGGGGGGGGG25380.13508507485565377No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTG3150.014.0071651
CTCCTTT1300.014.0071641
CGCCTTT300.005916989414.0071641
CGCCTTG300.005916989414.0071641
TTCCGTG453.0205125E-514.0071641
TGCCGTG300.005916989414.0071641
CGCCTGT652.7794158E-814.0071641
TTCCGGG704.8676156E-914.0071641
TGCCGGT300.005916989414.0071641
GTCCGTT453.0205125E-514.0071641
CCTCGTT453.0205125E-514.0071641
CCTCGTG453.0205125E-514.0071641
GGCCGTT350.001014437614.0071641
GGCCGTG505.239761E-614.0071641
GTCCGGG505.239761E-614.0071641
GGCCGGT350.001014437614.0071641
TCGCGTT505.239761E-614.0071641
CCGCTGT453.0205125E-514.0071641
GCGCGGT300.005916989414.0071641
TGCCTTG505.239761E-614.0071641
Produced by FastQC (version 0.11.5)