FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876469
Sequences flagged as poor quality0
Sequence length20
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT62000.33040780316647916No Hit
GGGTTTGGGTTTGGGTTTGG59550.31735136578328765No Hit
GGGGGGGGGGGGGGGGGGGG24970.1330690781462417No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGT3250.014.007121
GTCCTGG2900.014.007121
CTCCTGT2650.014.0071191
TGCCGTG401.747105E-414.0071191
CTCCTGG1600.014.0071191
GTCCGTT1050.014.0071191
CCTCGTG1100.014.0071191
GGCCGTG401.747105E-414.0071191
GTCCGGT350.001014461414.0071191
CCTCGGT401.747105E-414.0071191
CCTCGGG559.11632E-714.0071191
TTCCTTT1350.014.0071191
TGCCTTT559.11632E-714.0071191
TTCCTGG1950.014.0071191
TGCCTGT1250.014.0071191
TGCCTGG1000.014.0071191
CTGCGTT401.747105E-414.0071191
CCTCTTT1100.014.0071191
CCTCTTG1250.014.0071191
GGCCTTT1050.014.0071191
Produced by FastQC (version 0.11.5)