FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n01_dpr89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n01_dpr89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727204
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGT52770.30552268290254075No Hit
GGGTTTGGGTTTGGGTTTGG38270.22157197412697055No Hit
GTGGTTGTGTGGTGTTTTGT26930.15591673016042112No Hit
GGGGGGGGGGGGGGGGGGGG23900.13837392687835368No Hit
GTGGTTTTGTGTGTTTTTGT21660.12540498979854148No Hit
GTGGTTGTGTGTGGTTTTGT18180.10525681969240461No Hit
GTGTTGGTCGTGGTTTTTGG18010.10427257000331172No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT559.088071E-714.0108081
CCTCGTG559.088071E-714.0108081
TTCCTTT1100.014.0108081
TGCCTGT1100.014.0108081
CTGCGTG559.088071E-714.0108081
GGCCTTT559.088071E-714.0108081
GGCCTGG1100.014.0108081
CTTCGGT559.088071E-714.0108081
CTTCGGG559.088071E-714.0108081
CCCCTGG1100.014.0108081
TCTCTGT2200.014.0108081
GCTCTTG1500.014.0108081
CTTCTGT2900.014.0108081
GCCCTGT1100.014.0108081
CTCCTTT1050.014.0108071
CTCCTTG1350.014.0108071
TTCCGTG350.001012387314.0108071
CTCCTGT1950.014.0108071
CTCCTGG1600.014.0108071
TTCCGGT453.0128433E-514.0108071
Produced by FastQC (version 0.11.5)